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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

Question 1

Histopathological examination of lung tissue from an immunocompromised patient reveals large (8-15 µm), spherical yeast cells with thick, refractile walls and characteristic single, broad-based budding. These findings are most suggestive of infection with:

A. Coccidioides immitis/posadasii
B. Paracoccidioides brasiliensis
C. Histoplasma capsulatum
D. Blastomyces dermatitidis

Question 2

Transmissible spongiform encephalopathies (TSEs), such as Creutzfeldt-Jakob disease (CJD) in humans and Bovine Spongiform Encephalopathy (BSE) in cattle, are caused by infectious agents composed primarily of:

A. Defective viral particles requiring a helper virus
B. Slow-growing bacteria lacking a cell wall
C. Viroids (small, circular RNA molecules)
D. Aberrantly folded host-encoded prion proteins (PrP<sup>Sc</sup>)

Question 3

A clinical microbiology laboratory is performing Gram staining on a sputum sample. After adding the crystal violet stain, a delay in the washing step with decolorizer (alcohol-acetone) would MOST likely result in:

A. Poor adherence of the stain to Gram-positive bacteria.
B. Increased background staining.
C. Gram-positive bacteria appearing pink.
D. Gram-negative bacteria appearing purple.

Question 4

A clinical microbiology laboratory is evaluating a new rapid diagnostic test for influenza viruses. The test has a high sensitivity but a lower specificity compared to RT-PCR. In a setting with a low prevalence of influenza, the use of this rapid test is MOST likely to result in a high number of:

A. True positive results
B. False negative results
C. False positive results
D. True negative results

Question 5

Clavulanic acid is often combined with amoxicillin (e.g., Augmentin™) to overcome certain types of bacterial resistance. What is the primary mechanism by which clavulanic acid enhances amoxicillin's activity?

A. It functions as a potent, irreversible inhibitor of many bacterial beta-lactamase enzymes.
B. It increases the outer membrane permeability to amoxicillin in Gram-negative bacteria.
C. It binds to alternative penicillin-binding proteins (PBPs), providing a synergistic bactericidal effect.
D. It inhibits bacterial efflux pumps that would otherwise remove amoxicillin from the cell.

Solutions: