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American Society of Microbiology ABMM Sample Questions:

1. A patient with a history of recurrent Clostridioides difficile infection (CDI) is being considered for fecal microbiota transplantation (FMT). Pre-FMT screening of the donor stool sample should include comprehensive testing to exclude potential transmission of enteric pathogens. Which of the following would be the MOST critical to rule out in the donor stool?

A) Quantification of the overall microbial diversity using 16S rRNA gene sequencing.
B) Evidence of asymptomatic carriage of multidrug-resistant organisms (MDROs) such as vancomycin- resistant enterococci (VRE) or carbapenem-resistant Enterobacterales (CRE).
C) Detection of common antibiotic resistance genes not currently expressed.
D) Presence of non-toxigenic C. difficile strains.

2. A microbiology laboratory is performing antimicrobial susceptibility testing using Etest strips. The MIC value is determined by:

A) The presence or absence of growth at a single critical concentration.
B) The lowest concentration at which the elliptical zone of inhibition intersects the strip.
C) The diameter of the zone of inhibition around the strip.
D) The highest concentration that allows visible bacterial growth within the zone of inhibition.

3. A clinical microbiology laboratory is performing antimicrobial susceptibility testing on a Klebsiella pneumoniae isolate. The isolate is resistant to multiple beta-lactam antibiotics, and further testing reveals the presence of a carbapenemase enzyme. The MOST clinically significant carbapenemase in Klebsiella pneumoniae is often:

A) Klebsiella pneumoniae carbapenemase (KPC)
B) AmpC beta-lactamase
C) Metallo-beta-lactamase (MBL)
D) Extended-spectrum beta-lactamase (ESBL)

4. A patient develops a severe diarrheal illness after returning from a backpacking trip where they drank untreated stream water. Stool examination reveals motile trophozoites with multiple flagella and a prominent ventral sucking disk. The MOST likely causative agent is:

A) Giardia lamblia
B) Cryptosporidium parvum
C) Entamoeba histolytica
D) Cyclospora cayetanensis

5. A clinical microbiology laboratory is performing quality control on antimicrobial susceptibility testing. The zone diameter for Escherichia coli ATCC 25922 tested against ciprofloxacin is consistently smaller than the acceptable range published by CLSI. The MOST likely cause of this discrepancy is:

A) Inoculation with a bacterial suspension that is too dense
B) Incubation at an incorrect temperature
C) Use of expired antibiotic disks
D) All of the above

Solutions: